pegfp c1 eralpha Search Results


pegfp c1 eralpha  (Addgene inc)
93
Addgene inc pegfp c1 eralpha
Pegfp C1 Eralpha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+eralpha/bio_rxiv__2023__10__16__562466-239-18-19?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pegfp c1 eralpha - by Bioz Stars, 2026-08
93/100 stars
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erα  (Addgene inc)
94
Addgene inc erα
Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with <t>ERα</t> (A, C) <t>or</t> <t>ERβ</t> (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).
Erα, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+eralpha/pm27454708-130-6-8?v=Addgene+inc
Average 94 stars, based on 1 article reviews
erα - by Bioz Stars, 2026-08
94/100 stars
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esr1  (Addgene inc)
93
Addgene inc esr1
Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with <t>ERα</t> (A, C) <t>or</t> <t>ERβ</t> (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).
Esr1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+eralpha/pmc11670210__pnas%2E2408049121%2Esapp-29-11-22?v=Addgene+inc
Average 93 stars, based on 1 article reviews
esr1 - by Bioz Stars, 2026-08
93/100 stars
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pcdna3 prb  (Addgene inc)
93
Addgene inc pcdna3 prb
Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with <t>ERα</t> (A, C) <t>or</t> <t>ERβ</t> (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).
Pcdna3 Prb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+eralpha/bio_rxiv__2023__10__16__562466-239-22-23?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pcdna3 prb - by Bioz Stars, 2026-08
93/100 stars
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rd generation lentiviral vector pljm1 egfp  (Addgene inc)
96
Addgene inc rd generation lentiviral vector pljm1 egfp
Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with <t>ERα</t> (A, C) <t>or</t> <t>ERβ</t> (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).
Rd Generation Lentiviral Vector Pljm1 Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+c1+eralpha/bio_rxiv__2023__10__16__562466-239-42-47?v=Addgene+inc
Average 96 stars, based on 1 article reviews
rd generation lentiviral vector pljm1 egfp - by Bioz Stars, 2026-08
96/100 stars
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Image Search Results


Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with ERα (A, C) or ERβ (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).

Journal: Planta medica

Article Title: Estrogenic Activity of Hyperforin in MCF-7 Human Breast Cancer Cells Transfected with Estrogen Receptor.

doi: 10.1055/s-0042-112594

Figure Lengend Snippet: Fig. 1 Effects of St Johnʼs wort (SJW) extract, hyperforin (HF), and 17β- estradiol (17β-E2) on ERE-luciferase activity in MCF-7 cells. The luciferase activities in MCF-7 cells transfected with ERα (A, C) or ERβ (B, D) and an ERE reporter plasmid were measured after treatment with 0.2 to 20 µg/mL SJW extract (A, B) or 10−5 to 10 µM HF (C, D). A positive control was prepared by treating the cells with 10−2 µM 17β-E2. Relative luciferase activities were calculated as percentages of the induced luminance relative to control after normalization to the Renilla luciferase activity. Bars represent the averages of triplicate determinations. Asterisks indicate significant differences from the control (Ctrl) determined using Dunnettʼs multiple comparison t-test (*p < 0.05 and *** p < 0.001).

Article Snippet: Briefly, Opti-MEM was used to dilute ERα (pEGFP‐C1-ERα, Addgene), ERβ (pcDNA Flag ERβ, Addgene), ERE (3× ERE TATA luc, Addgene), and Renilla luciferase (pRL-SV40, Promega) prior to the addition of the transfection reagent.

Techniques: Luciferase, Activity Assay, Transfection, Plasmid Preparation, Positive Control, Control, Comparison